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Ensuring successful protein expression through proper plasmid purification

SOLVED: Give a short discussion for each of the reagents of the plasmid isolation procedure. Include the step the reagent is associated, and the function of the reagent (in relation to the DNA/proteins/ or other chemicals)* Note- not all the reagents are provided for each buffer/solution used in the plasmid Isolation experiment. Include the resource(s) that you use to help answer this question. 70% Ethanol - 200mM sodium Hydroxide/1% SDS -

Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis