Ensuring successful protein expression through proper plasmid purification

SOLVED: Give a short discussion for each of the reagents of the plasmid isolation procedure. Include the step the reagent is associated, and the function of the reagent (in relation to the DNA/proteins/ or other chemicals)* Note- not all the reagents are provided for each buffer/solution used in the plasmid Isolation experiment. Include the resource(s) that you use to help answer this question. 70% Ethanol - 200mM sodium Hydroxide/1% SDS -

Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

Plasmid Vector Construction

Difference between Plasmid and Vector

Plasmid Cloning by PCR

the plasmid vector you used for cloning has three ecori sites as shown on the plasmid diagram and one bamhlsite located within the polylinker multiple cloning site the numbers in kb kilobase 00676

Cloning - Definition and Examples - Biology Online Dictionary