2001年12月25日 · Here we report a detailed characterization of a reliable and efficient chemical-inducible system, termed XVE for LexA-VP16-ER. The XVE activator is strictly regulated by estradiol in transgenic plants, with undetectable transactivating activity in …

We have developed an estrogen receptor-based chemical-inducible system for use in transgenic plants. A chimeric transcription activator, XVE, was assembled by fusion of the DNA-binding domain of the bacterial repressor LexA (X), the acidic transactivating domain of VP16 (V) and the regulatory region …

2013年11月1日 · Here we describe a new set of 57 vectors that enable transgene expression in transiently or stably transformed cells. All vectors harbor a synthetically optimized XVE expression cassette, allowing β-estradiol mediated protein expression.

• The XVE system is not toxic to the plants • The system is reliable, efficient, and specific for estrogen inducers. • Target genes are induced without pleiotropic effects. • The system responds to a broad range of inducer concentrations in a dose-dependent manner, therefore allowing stringent chemical control of transcription.

The LexA-VP16-ER (XVE) system is an estrogen-receptor-based chemical-inducible system for use in plants. Protocols are outlined for the inducible overexpression of transgenes, inducible transgene excision, inducible gene silencing, regulated in vivo …more

2001年12月25日 · We have developed an estrogen receptor-based chemical-inducible system for use in transgenic plants. A chimeric transcription activator, XVE, was assembled by fusion of the DNA-binding domain of the bacterial repressor LexA (X), the acidic transactivating domain of VP16 (V) and the regulatory region of the human estrogen receptor (E; ER).

2017年8月20日 · Here, we constructed an improved chemical-regulated, site-specific recombination system by employing the XVE transactivator in combination with a Cre/loxP-FRT system, and optimized a seed-soaking procedure for XVE induction in rice.

Before induction, a constitutive ubiquitin promoter Pubi drives the expression of the chimeric transcriptional activator XVE. When XVE is activated by the estrogen inducer, it stimulates the target promoter Olex-46 to express the Cre DNA recombinase.

2013年9月27日 · Establishment of an estrogen-inducible gene expression system in P. patens. We modified the A. thaliana XVE system by changing the original promoter driving XVE to a promoter effective in P. patens and obtained reproducible amounts of transcript from a transactivated gene by β-estradiol application.

2015年12月7日 · Here, we introduce a MultiSite Gateway-compatible inducible system for Arabidopsis (Arabidopsis thaliana) plants that provides the capability to generate such constructs in a single cloning step. The system is based on the …