2024年5月15日 · The reference standard serologic test for diagnosis of anaplasmosis is the indirect fluorescent antibody (IFA) test for immunoglobulin G (IgG) using Anaplasma phagocytophilum antigen. IgG IFA tests should be performed on paired acute and convalescent serum samples, with the acute specimen collected during the first two weeks of illness and the ...

2023年8月8日 · Microscopic examination of peripheral blood smear shows characteristic intracytoplasmic aggregates in neutrophils called morulae. This finding is detected 25% to 75% of the time in patients that have not begun therapy. The sensitivity of peripheral smear for diagnosis is higher during the first week of infection.

Diagnosis of anaplasmosis is confirmed by finding the parasite – it is recognized as small, round dark purple inclusions located at the periphery of red cells. There are usually more than 1-2 per cell. The organism must be differentiated from Howell-Jolly bodies.

HGA is a tick-borne illness caused by Anaplasma phagocytophilum and it is important to distinguish from other tick-borne diseases. Detection of morulae in the peripheral blood smear can provides a rapid diagnostic clue for HGA.

Serologic testing is useful to confirm the diagnosis of anaplasmosis. The most commonly used method is indirect immunofluorescence (IFA) of IgM and IgG anti- A. phagocytophilum antibodies.

Rarely, intra-granulocytic or monocytic morulae may be observed on peripheral blood smear, but this is not a reliable means of diagnosing cases of human ehrlichiosis or anaplasmosis. Definitive diagnosis is usually accomplished through polymerase chain reaction (PCR) and …

2013年11月12日 · A complete blood count on presentation showed pancytopenia (WBC 2.6, Hgb/Hct 10.5/29.9, Plt 113). The peripheral blood smear showed rare neutrophils with single basophilic intracytoplasmic inclusions (pictured).

Anaplasma infection can be detected via ELISA or IFA testing. Such serological testing can identify dogs with prior exposure but is prone to cross-reactivity and not very sensitive. Examination of blood smears can detect the pathogen but …

Objectives: We sought to assess the performance of 3 laboratory tests on blood specimens for direct detection of Anaplasma phagocytophilum, the cause of human granulocytic anaplasmosis (HGA), in patients tested at a single medical institution in New York State.

We compared the performance of a published real-time PCR assay that incorporates melt curve analysis to differentiate Anaplasma and Ehrlichia species with blood smear and serologic methods in an upper Midwest population. Overall, 38.5% of the specimens selected for evaluation had one or more tests that were positive for anaplasmosis.