2023年12月13日 · Here we report a comprehensive and high-resolution transcriptomic and spatial cell-type atlas for the whole adult mouse brain. The cell-type atlas was created by combining a single-cell...

2021年7月28日 · A comprehensive single-cell transcriptomic atlas of the mouse brain between gastrulation and birth identifies hundreds of cellular states and reveals the spatiotemporal organization of...

2024年12月18日 · Tiklová, K. et al. Single-cell RNA sequencing reveals midbrain dopamine neuron diversity emerging during mouse brain development. Nat. Commun. 10, 581 (2019).

2023年1月5日 · We performed snRNA-seq measurements to probe the transcriptomic profiles of individual cells from the frontal cortex and striatum of mice at two different ages, ∼1 month (4-week-old, juvenile) and ∼21 months (90-week-old, old) postnatal (Figures 1 A and 1B).

2018年4月13日 · To facilitate scalable profiling of single cells, we developed split-pool ligation-based transcriptome sequencing (SPLiT-seq), a single-cell RNA-seq (scRNA-seq) method that labels the cellular origin of RNA through combinatorial barcoding. SPLiT-seq is compatible with fixed cells or nuclei, allows e …

Single cell (or single nucleus) RNA sequencing (RNA-Seq) is a scalable approach to provide genome-wide expression profiles for thousands of cells. This data set includes single cell and nuclear transcriptomic profiles, assayed from human and mouse brain regions.

2018年3月15日 · We introduce split-pool ligation-based transcriptome sequencing (SPLiT-seq), a low-cost, scRNA-seq method that enables transcriptional profiling of hundreds of thousands of fixed cells or nuclei in a single experiment.

2017年3月28日 · Here, we report single-cell RNA sequencing results of adult mouse hypothalamus, which defines 11 non-neuronal and 34 neuronal cell clusters with distinct transcriptional signatures. Analyses of cell-type-specific transcriptomes reveal gene expression dynamics underlying oligodendrocyte differentiation and tanycyte subtypes.

2022年5月20日 · We survey published single-cell RNA-sequencing studies of mouse and human brain development, organized by anatomical regions and developmental time points. We highlight remaining gaps in the field, predominantly concerning human brain development.

2019年1月15日 · Here, we analyzed the RNA expression patterns of more than 76,000 individual microglia in mice during development, in old age, and after brain injury. Our analysis uncovered at least nine transcriptionally distinct microglial states, which expressed unique sets of genes and were localized in the brain using specific markers.