Bacterial expression vector with a BamHI cloning site. For alternative reading frames, use pET-11b or pET-11c.

Plasmid Protocol. 1. Centrifuge at 5,000×g for 5 min. 2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature.

Plasmid pET11a_PTK2b_Kinase from Dr. Andrew Mesecar's lab contains the insert ptk2b_kinase. This plasmid is available through Addgene. Image: Illustrated plasmid map in PNG format. GenBank File: Plasmid sequence and annotations. Use text editor or plasmid mapping software to view sequence.

2011年9月16日 · sequence. the sequence provided is not compatible with restriction maps or vector information obtained prior to december, 2001. 1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta 51 gaaataattt tgtttaactt taagaaggag atatacatat ggctagcatg 101 actggtggac agcaaatggg tcgcggatcc ggctgctaac aaagcccgaa 151 aggaagctga gttggctgct gccaccgctg …

T7 promoter with lac operator 1–43 ribosome binding site 74–80 Nde I cloning site 86–91 T7 gene 10 leader 89–121 BamH I cloning site 125–130 T7 terminator 199–245

pET-11a DNA - Novagen Novagen's pET-11a-d vectors carry an N-terminal T7-Tag sequence and BamH I cloning site. The pET-21a-d(+) series corresponds to pET-11a-d but incorporates several additional features. - Find MSDS or SDS, a COA, data sheets and more information.

pET System Vectors and Hosts Instruction Manual Catalog #211521, #211523, #211621, #211623 Revision C0 For Research Use Only. Not for use in diagnostic procedures.

Plasmid sequence and annotations. Use text editor or plasmid mapping software to view sequence. SnapGene File: Plasmid sequence and SnapGene enhanced annotations. Use with SnapGene software or the free Viewer to visualize additional data and align other sequences.

A repository of over 200,000 plasmids including Protein Structure Initiative protein expression plasmids and vectors, over 75,000 human plasmids, and whole genome collections from many organisms.

Plasmid pET11a-link-NGFP from Dr. Lynne Regan's lab contains the insert link-NGFP and is published in J Am Chem Soc. 2005 Jan 12;127(1):146-57. This plasmid is available through Addgene. Image: Illustrated plasmid map in PNG format. GenBank File: Plasmid sequence and annotations. Use text editor or plasmid mapping software to view sequence. ...