2015年12月1日 · Imlay and Linn show that exposure of logarithmically growing Escherichia coli to hydrogen peroxide (H 2 O 2) leads to two kinetically distinguishable modes of cell killing. Mode one killing is pronounced near 1 mM concentration of H 2 O 2 and is caused by DNA damage, whereas mode-two killing requires higher concentration ( > 10 mM ).

Hydrogen peroxide (H2O2) is an oxidizing agent that induces cellular damage at inappropriate concentrations and gives rise to an arrest during cell cycle progression, causing cell death. Recent evidence indicates that H2O2 also acts as a promoter ...

Cell killing and DNA damage by hydrogen peroxide are mediated by intracellular iron. Phenanthroline, a strong iron chelator, prevents both the formation of DNA single-strand breaks and the killing of mouse cells produced by H2O2.

2016年8月5日 · In this experiment, whole cells seemed to scavenge H 2 O 2 less efficiently than cell extracts. The cell membrane slows the entry of H 2 O 2, resulting in lower rates of decomposition. It also protects cells against high H 2 O 2 concentrations, by decreasing the maximum value of H 2 O 2 concentration. This phenomenon is described in more detail ...

Hydrogen peroxide (H 2 O 2) is an important intra- and extra-cellular signaling molecule that can determine cell fate. At low concentrations, H 2 O 2 plays roles in proliferation, immunity, and metabolism. Cellular exposure to higher non-physiologic concentrations of H 2 O 2 can result in oxidative stress. If the stress is not alleviated, cell ...

The studies reported here investigate H2O2-induced cell death in more detail. Our data suggest that exposure of cultured cortical neurons to H2O2 can induce apoptotic cell death within 3 h, as assessed by cell viability, morphological and ultrastructural measures.

2011年10月6日 · Exposure to pathogenic organism triggers Nox2 complex assembly in the neutrophil by recruiting various subunits to the plasma membrane. The activated Nox complex then releases superoxide in...

2016年8月5日 · The external H2 O2 half-life and the cumulative internal H2 O2 exposure do depend upon cell density. Based on these analyses and in order to introduce a concept of dose response relationship for H2 O2-induced cell death, we developed the concepts of "maximal internal H2 O2 concentration" and "cumulative internal H2 O2 concentration" (e.g. the ...

2020年3月26日 · Hydrogen peroxide (H 2 O 2) is an oxidizing agent that induces cellular damage at inappropriate concentrations and gives rise to an arrest during cell cycle progression, causing cell death. Recent evidence indicates that H 2 O 2 also acts as a promoter for cell cycle progression by oxidizing specific thiol proteins.

Extensive research has focused on selective generation of H 2 O 2 in cancer cells for selective cancer cell killing by employing various strategies such as metal-based prodrugs, photodynamic therapy, enzyme-based systems, nano-particle mediated approaches, chemical modulators, and combination therapies.