In brief, with this article we bring light to new findings concerning the role of hydroxyl radical in guanine oxidation, present a few mysteries regarding the identities of major oxidation products of guanine, and discuss the impact of guanine oxidation on gene expression via DNA repair.

2022年10月21日 · In pathophysiology, reactive oxygen species control diverse cellular phenotypes by oxidizing biomolecules. Among these, the guanine base in nucleic acids is the most vulnerable to producing...

Emerging evidence suggests the modified base 8-oxoguanine, created when damaging chemicals called reactive oxygen species interact with DNA and RNA, has more extensive effects on cell function than previously suspected.

By coupling the two techniques (the cryogenic stabilization and the time-resolved laser flash photolysis spectroscopy) together, we are able to capture the ion-pair transient G +• ⋯Cl − in the chlorine radical–initiated DNA guanine (G) oxidation reaction, and provide direct evidence to ascertain the intricate type of addition/charge ...

Guanine is the most readily oxidized of the four DNA bases, and guanine oxidation products cause G:C-T:A and G:C-C:G transversions through DNA replication. 8-Oxo-7,8-dihydroguanine (8-oxoG) causes G:C-T:A transversions but not G:C-C:G transversions, and is more readily oxidized than guanine.

Purpose: One outcome of DNA damage from hydroxyl radical generated by ionizing radiation (IR) or by the Fenton reaction is oxidation of the nucleobases, especially guanine (G). While 8-oxo-7,8-dihydroguanine (OG) is a commonly studied oxidized lesion, several others are formed in high abundance, including 5-carboxamido-5-formamido-2 ...

2006年7月26日 · This article analyses the mechanisms underlying guanine oxidation by the comparison between one- and two-electron transfer processes. The different oxidants used in vitro give complementary answers. This overview presents a choice of some key intermediates and the predictive description of G-oxidation products that can be generated from these ...

2020年10月15日 · The damage caused by oxidation of guanine, commonly referred to as oxidative guanine damage, results in the formation of several products, including 2,5-diamino-4H-imidazol-4-one (Iz), 2,2,4-triamino-5(2H)-oxazolone (Oz), guanidinoformimine (Gf), guanidinohydantoin/iminoallantoin (Gh/Ia), spiroiminodihydantoin …

Under physiological conditions, ONOO – reacts with CO 2 to form nitrosoperoxycarbonate (ONOOCO 2–) which homolyzes to form • NO 2 and CO 3•–, and the latter oxidizes the guanine (Gua) nucleobase [ 3 ]. 1 O 2 is also able to oxidize Gua whereas • OH reacts with all four nucleobases and also the 2′-deoxyribose sugar ring [ 4 ].

2012年4月20日 · Hydroxyl radicals are powerful oxidizing agents in living cells and will oxidize the nucleic acid base, guanine, to form 8-oxoguanine, which is potentially mutagenic because it can pair with both cytosine and adenine and form lethal double-strand DNA breaks.