Live-cell imaging is the study of living cells using time-lapse microscopy. It is used by scientists to obtain a better understanding of biological function through the study of cellular dynamics. [1] Live-cell imaging was pioneered in the first decade of the 21st century.

These live-cell imaging setups provide a powerful tool to study dynamic cellular processes on a single cell level. Aside from giving a direct readout of cellular physiology and behavior, fluorescence microscopy can be used to simultaneously obtain insight into molecular processes.

There are 16 new chapters and 21 updated chapters in this new edition. They include advances in atomic force microscopy, structured illumination microscopy and other 3-D approaches, as well as imaging in single cells in animals and in plants.

2024年2月20日 · In this Review, we discuss these computational methods focusing on artificial intelligence-based approaches that can be layered on top of commonly used existing microscopies as well as hybrid...

There are many factors that need to be considered when performing live-cell imaging in order to maintain cellular health such as: imaging modality, media, temperature, humidity, PH, osmolality, and photon dose.

Live cell imaging systems are specialized instruments that include a microscope, environmental control and image acquisition software. This combination allows cells to maintain viability while being imaged over a long period of time.

2024年2月27日 · Live-cell imaging integrates advanced microscopy techniques, such as fluorescence and confocal microscopy, with the capacity to maintain cells in a life-supporting state and elevates the study of cells beyond traditional microscopy’s capabilities.

Overall, live cell imaging represents a powerful alternative to fixed cell imaging, and with recent advances and technologies such as quantitative phase-contrast and live fluorescence, more researchers are imaging their cells live in order to get the most biologically relevant data.

2012年8月21日 · Imaging live-cells using transmitted light is often used with fluorescence microscopy to determine cell shape, motility, and position. This is important for investigating cells that undergo changes in shape, such as mitosis and apoptosis.

The application of genetically-encoded fluorescent proteins has dramatically expanded the capabilities of live-cell imaging in fluorescence microscopy by enabling investigators to target subcellular regions of interest with exacting precision.

2024年2月13日 · Super-Resolution Microscopy transforms Live-Cell Imaging, offering unprecedented glimpses into cellular mechanisms with nanoscale precision. This cutting-edge technique is revolutionizing the understanding of biological processes, paving the way for breakthroughs in medical research.

2003年4月4日 · Imaging living cells with transmitted light is often used along with fluorescence microscopy in order to provide information on cell shape, position, and motility. This is absolutely vital when studying processes such as apoptosis and …

Explore Nikon's high-speed solution for maintaining stable focus in live-cell imaging. Learn how to adjust an objective correction collar to minimize spherical aberration. Identification of critical filter parameters for imaging fluorescent proteins. Interactive tutorial used to optimize pairing of two fluorescent proteins.

Live Cell Imaging is a method to optically investigate living cells over a period of time using time-lapse microscopy. Thus, processes such as fertilization and cellular development, cell signaling, cell migration or cell differentiation can be envisioned and comprehended.

Super-resolution confocal live imaging microscopy (SCLIM), which we have developed (Kurokawa et al., 2013), can achieve high speed visualization of dynamic behavior of 3D structures tagged with green and red fluorescence proteins in living cells with spatial resolution beyond the diffraction limit of light (Matsuura-Tokita et al., 2006; Ito et ...

2024年3月14日 · Live cell imaging is an analytical microscopy-based technique that researchers use to observe living cells over several seconds, hours, or even days. 1 Through time-lapse microscopy, scientists track proteins, organelles, individual cells, or cell populations during dynamic processes, such as phagocytosis, wound healing, or differentiation.

2022年4月21日 · Live-cell microscopy is a powerful tool that can reveal cellular behavior as well as the underlying molecular processes. A key advantage of microscopy is that by visualizing biological processes, it can provide direct insights.

What is live cell imaging? To ensure successful time-lapse observation of live cells, your microscope system must maintain constant environmental control, suppress any phototoxicity and photobleaching, and provide stable imaging.

5 天之前 · In long-term live-cell imaging experiments, cells were held in a stage-top incubator equipped on the microscope (OkO Lab, H301) to maintain conditions at 37 °C with 5% CO 2. We used the TIRF/GI ...

2022年4月21日 · These live-cell imaging setups provide a powerful tool to study dynamic cellular processes on a single cell level. Aside from giving a direct readout of cellular physiology and behavior, fluorescence microscopy can be used to simultaneously obtain …

2025年1月23日 · Super-resolution microscopy is an optical imaging technology that overcomes the diffraction limit of light and allows the visualization of subcellular structures and dynamics in greater detail than can be achieved with conventional optical microscopy. ... Extended live-cell imaging with TauSTED Xtend. Combined spatial and lifetime information ...

2022年10月4日 · In this article, we name five good reasons why it makes sense to study living cells with time-lapse microscopy. Live cell imaging allows the in vivo observation of cells under the microscope over a certain period. Various microscopy techniques are suitable for live cell imaging.

Equipment: Leica SP8 Confocal, STED, FLIM System: The Leica SP8 confocal microscope is for live cell imaging with high-speed, multi-spectral, super-sensitivity, super-resolution, and fluorescence lifetime capability. Olympus FV1000 Confocal Microscope: Confocal microscope capable of optical sectioning and 3D imaging of fluorescently labeled samples. . Both live …

5 天之前 · While plant researchers typically prefer upright stands for their microscopy experiments, the CAi has only inverted microscope stands. This compromise in instrument configuration is necessary because many biomedical researchers also use the CAi systems to perform experiments such as live cell imaging with the confocal microscopes.

2025年1月24日 · Fluorescence microscopy. The live-cell DNA imaging was carried out on a DeltaVision Ultra imaging system equipped with a 100× PlanApo oil objective lens (NA 1.4). The cells were cultured on No. 1.0 glass bottom dishes (MatTek). BFP was excited with an excitation filter at 397/31 nm, and its emission was collected using an emission filter at ...

Super-resolution (SR) neural networks transform low-resolution optical microscopy images into SR images. Application of single-image SR (SISR) methods to long-term imaging has not exploited the temporal dependencies between neighboring frames and has been subject to inference uncertainty that is dif …

2 天之前 · Influenza A virus structure and the inverted attachment approach. (a) Structural organization of influenza A virus (IAV) particles.(b) Electron microscopy image of influenza virus A/Puerto Rico/8/1934 (H1N1) particles (Image courtesy of Dr. Mathias Müsken, HZI Braunschweig).(c) Inverted attachment approach.Immobilized viral particles in combination …

4 天之前 · Fluorescence lifetime imaging microscopy (FLIM) has revolutionized bioimaging by introducing a layer of information that surpasses the capabilities of conventional intensity-based imaging methods. ... “Graphene- and metal-induced energy transfer for single-molecule imaging and live-cell nanoscopy with (Sub)-Nanometer axial resolution,” Nat ...

3 天之前 · For live-cell imaging of socRNAs, the x, y positions for imaging were chosen based on the presence of translated socRNAs in cells. Images were acquired every 90-180 sec for 1-4 hours with exposure times for the 488 nm laser ranging from 50-100 ms.

2025年1月10日 · cost-effective, low-maintenance, confocal microscopy, live cell imaging, heat stress, heat shock protein, stress granules (SG), microheater. Frontiers in Plant Science frontiersin.org 01. OPEN ACCESS.

3 天之前 · Scientists have developed a breakthrough microscopy technique to observe ribosomes in action as they translate mRNA into proteins. This method revealed that ribosomes cooperate when encountering obstacles, rather than just being removed by the cell’s quality control system. A new imaging technique ...

2025年1月16日 · Mitochondrial morphology and function are intrinsically linked, indicating the opportunity to predict functions by analyzing morphological features in live-cell imaging. Herein, we introduce MoDL, a deep learning algorithm for mitochondrial image segmentation and function prediction. Trained on a da …

4 天之前 · Light microscopy makes it difficult to resolve smaller and smaller features, and electron microscopy resolves small structures, but samples must be meticulously prepared, killing any live specimens.

3 天之前 · Beyond being the core control center for the cell, the nucleus is one of the most important targets in bioimaging research. 1 For example, looking at the nucleus can tell researchers about cell viability, DNA damage, cell cycle status, and localization of nuclear molecules. Fluorescent imaging allows researchers to peer into a cell’s inner workings, …