Depurination is a chemical reaction of purine deoxyribonucleosides, deoxyadenosine and deoxyguanosine, and ribonucleosides, adenosine or guanosine, in which the β-N- glycosidic bond is hydrolytically cleaved releasing a nucleic base, adenine or guanine, respectively.

2022年12月30日 · Depurination of guanines (or adenines) is a common DNA lesion. Three of the four DNA bases, adenine, guanine, and cytosine, contain amine groups that can be lost in a variety of pH and temperature-dependent reactions that convert the bases to hypoxanthine, xanthine, and uracil, respectively.

2014年12月29日 · Depurination, the release of purine bases from nucleic acids by the hydrolysis of N-glycosidic bonds, has aroused considerable interest for a long time because of its close relationship with mutation and repair of nucleic acids.

Depurination arises from spontaneous chemical reactions, typically hydrolysis, which breaks the labile glycosidic bonds between the DNA base and the deoxyribose creating an abasic site. These sites can result in mutations due to misincorporation by DNA polymerase or translesion DNA synthesis (TLS) [2]. Spontaneous, hydrolytic reactions such as ...

For instance, depurination (Figure 5), in which a purine base is lost from a nucleotide through hydrolysis even though the sugar-phosphate backbone is unaltered, can occur without an explicit ...

2018年1月1日 · Depurination is a term usually applied to the loss of a purine (which is more common) or a pyrimidine, leading to an abasic site (also called an apurinic site for the same reason). Abasic sites are the most common modification in the genome in most organisms, including humans.

Approximately 80% of the base-substitution mutations occur at purine positions in the viral strand, consistent with depurination as the predominant premutagenic lesion. The preference of guanine over adenine sites mutated is consistent with the preference for …

“Depurination” is a process in which the purine base of a DNA molecule is lost, potentially leading to a somatic mutation and carcinogenesis. Aflatoxins can damage the mitochondrial membrane by covalent binding to mitochondrial DNA and disruption of energy (ATP) production, and increased cell death by apoptosis.

Approximately 80% of the base-substitution mutations occur at purine positions in the viral strand, consistent with depurination as the predominant premutagenic lesion. The preference of guanine over adenine sites mutated is consistent with the preference for …

2016年7月30日 · We discuss the biochemistry of RNA depurination, the varied impacts of cellular and viral RNA depurination, and the numerous methods available for detecting abasic RNA. Given the abundance of RNA in cells and the relative stability of abasic RNA compared with DNA, depurinated RNA likely has physiological significance.