2017年5月1日 · The three-dimensional model was exposed to different conditions to mimic inflammatory diseases, such as dry eye or allergic conjunctivitis. Compared to controls, the increased MUC5AC secretion occurred in partially desiccated and IL-13-treated cultures.

2024年1月16日 · Scientists have created the first 3D model of the human conjunctiva — the clear, protective outer membrane of the eye — in the lab, and it even produces its own tears. The researchers say the...

2024年1月11日 · Here, we describe long-term expanding organoids and air-liquid interface representing mouse and human conjunctiva. A single-cell RNA expression atlas of primary and cultured human conjunctiva reveals that keratinocytes express multiple antimicrobial peptides and identifies conjunctival tuft cells.

Zuguo et al. proposed a new in vitro xeropthalmia model by dissecting the conjunctival epithelium and subconjunctival matrix, culturing it on a collagen I coated dish submerged in a culture medium. The in vitro dry eye model is obtained after 4–20 days.

2023年3月6日 · In this study, we compared the αβ TCR repertoire in the conjunctiva between desiccating stress animal models and controls. Some highly clonally expanded T-cell clonotypes with marked gene usage were investigated. This study aimed to contribute to the early diagnosis of dry eye by exploring possible TCR detection measures.

In this study, we developed a full thickness in vitro conjunctiva model that includes a multi-layer epithelium and mucus-secreting goblet cells. We used isolated primary epithelial cells from human conjunctival biopsies.

2016年3月20日 · To assess the conjunctival functionality in a rabbit dry eye (DE) model. Methods. Nictitating membrane, lacrimal and Harderian glands were surgically excised from male New Zealand white rabbits using minimally invasive surgery.

However, in this review, we discussed several models of the human cornea, conjunctiva, lens, etc., which may be combined to obtain a more sophisticated model of the anterior eye. In this article, we reviewed the main 3D in vitro models that have been developed to study human anterior eye tissues.

2024年1月19日 · Researchers create organoid model of human conjunctiva . Dutch scientists hope that the organoid model could be used to test drugs for a range of diseases that affect the conjunctiva ... Bannier-Hélaouët shared that the model could be used to test drugs for diseases that affect the conjunctiva, including allergies or dry eye disease. Comments ...

Culturing of eye tissues in microfluidic systems helps identify the toxic effects and pharmacological activity of new compounds, and provides an opportunity for deeper understanding of the normal...