Primary cilia can be distinguished nicely by differential interference contrast (DIC) microscopy when a microscope is properly aligned (see protocol described in (Salmon and Canman, 2001)). Wide-field and confocal imaging of immunostained cilia are commonly used to assess cilia formation and protein localization.

Samples can be viewed immediately under a microscope with an integrated camera capable of recording video at high speeds (120–500 frames/s), which can later be reviewed in slow motion for three-dimensional analysis of ciliary function, including both …

Cilia are microscopic, hair-like structures that project from the surface of many eukaryotic cells. Like other organelles of eukaryotic cells, cilia are membrane-bound structures with their membrane being continuous with the plasma membrane.

2015年11月13日 · A microscope also facilitates the observation of the tiny hairlike cilia that line the cells. Found most heavily along the respiratory tract, pseudostratified ciliated columnar epithelial cells help trap and transport particles brought in through the nasal passages and lungs.

2017年8月17日 · We demonstrate the development of a high-speed Doppler optical coherence tomography system that not only can rapidly determine the cilia beat frequency, but also simultaneously visualize the...

Place a drop of pond water under the microscope, and you will likely find an ocean of extraordinary and diverse single-celled organisms called ciliates. This remarkable group of single-celled organisms wield microtubules, active systems, electrical ...

2020年9月14日 · In this study, we show that SR imaging of primary cilia and centrioles is possible using proExM. We also demonstrate that observation of expanded samples by two types of SR microscopy, that is, structured illumination microscopy (SIM) and the Zeiss Airyscan, further improve the resolution.

2020年9月2日 · We developed a novel method to stain airway epithelial cilia to observe their movement without releasing ciliated cells. Human tracheae were obtained from patients (n = 13) who underwent...

2020年9月15日 · In this study, we applied ExM to the observation of primary cilia and centrioles and compared the acquired images with those obtained using conventional superresolution microscopy.

2015年8月18日 · This video shows a ciliate; a type of single-celled organism that inhabits a wide range of freshwater habitats. Ciliates feed upon smaller microscopic organisms, and move around by beating the...