Carbol fuchsin, carbol-fuchsin, carbolfuchsin, or Castellani's paint (CAS) is a mixture of phenol and basic fuchsin that is used in bacterial staining procedures. It is commonly used in the staining of mycobacteria because it has an affinity for the mycolic acids found in their cell membranes.

The primary stain used in acid fast staining, carbol fuchsin, is lipid-soluble and contains phenol, which helps the stain penetrate the cell wall. This is further assisted by the addition of heat in the form of heat (steam).

2024年11月11日 · Neelsen in 1883 used Ziehl’s carbol-fuchsin and heat then decolorized with an acid alcohol, and counter stained with methylene blue. Thus Ziehl-Neelsen staining techniques was developed. The main aim of this staining is to differentiate bacteria into acid fast group and non-acid fast groups.

2024年9月2日 · Carbol-Fuchsin is key for acid-fast bacteria stains (Ziehl-Neelsen, Kinyoun's, Fite) by resisting decolorization, detecting bacteria missed by Gram stains.

Carbol Fuchsin is first applied in the acid-fast method, which stains every cell. After destaining with acid alcohol, only nonacid-fast bacteria destain since they don’t have the thick, waxy lipid covering as acid-fast bacteria do.

2024年9月11日 · Carbol fuchsin is a superior stain for rose chromosomes, offering more effectiveness than Acetocarmine or Giemsa. It darkens chromosomes over time, making them ideal for detailed examination under a microscope. Carbol fuchsin outperforms Acetocarmine and Giemsa stains in visualizing rose chromosomes, ensuring clearer and more defined results.

2021年6月3日 · Acid-fast mycobacteria contain mycolic acid in their outer membrane, making the cells waxy and resistant to staining with aqueous based stains such as the Gram stain. The primary stain, carbolfuchsin is applied to the cells, and heat and phenol are used to allow the stain to penetrate into the waxy surface of acid-fast microorganisms.

2018年4月14日 · The Acid-Fast staining basically requires 3 reagents, A carbol Fuchsin Solution, An Acid Fast decolorizer and The Counter Stain, Methylene blue is commonly used. In this article, I’m gonna discuss the Preparation of All the Reagents require for the Acid Fast staining, in Laboratory. Let’s Start with Carbol Fuchsin preparation.

Carbol fuchsin, powder: Does not have an individual approval but may be used under an appropriate group standard

The Acid Fast Stain is a scientific method for identifying acid-fast microorganisms such as Mycobacterium tuberculosis. The theory behind this stain is based on particular bacteria’s capacity to maintain a red dye known as carbol fuchsin even after being treated to an acid-alcohol decolorizer. What is Acid Fast Stain?