2022年11月3日 · Carbamoyltransferases (CTases) catalyze carbamoylation, an important modification for the bioactivity of many secondary metabolites with pharmaceutical applications. To generate an iterative CTase,...

氨甲酰转移酶 (carbamoyltransferase)大肠杆菌嘧啶核苷酸从头合成途径中的限速酶，它催化氨甲酰磷酸和Asp形成N-氨甲酰天冬氨酸和无机磷酸，其活性受到严格的调控，以维持细胞内嘧啶核苷酸和嘌呤核苷酸比例的平衡.

Carbamylation is a post-translational modification in which cyanate binds to primary amino or thiol groups. The binding of cyanate to either amino groups or thiol groups is specified as N-carbamylation or S-carbamylation.

Aspartate carbamoyltransferase (also known as aspartate transcarbamoylase or ATCase) catalyzes the first step in the pyrimidine biosynthetic pathway (EC 2.1.3.2).

2022年11月7日 · 近日，上海交通大学生命科学技术学院白林泉教授团队在国际权威期刊《Nature Communications》上在线发表了题为“Endowing homodimeric carbamoyltransferase GdmN with iterative functions through structural characterization and mechanistic...

Here, we present the protocol to obtain purified 5-hydroxymethylcytosine carbamoyltransferase enzymes and the associated assays to convert 5-hydroxymethylcytosine to 5-carbamoyloxymethylcytosine in vitro . We include detailed assays using DNA, RNA, and single nucleotide/deoxynucleotide as substrates.

The carbamoyltransferase or aspartate carbamoyltransferase (ATCase)/ornithine carbamoyltransferase (OTCase) is an evolutionary conserved protein family, which contains two genes, ATCase and OTCase. The ATCase catalyzes the committed step in the synthesis of UMP from which all pyrimidine molecules are synthesized.

天冬氨酸转氨甲酰酶 （英語： Aspartate carbamoyltransferase， EC 2.1.3.2，简称为 ATCase）是 嘧啶核苷酸 从头合成 中很重要的一个 酶，它催化的是 天冬氨酸 与 氨甲酰磷酸 生成 氨甲酰天冬氨酸 和 磷酸 的反应。

天冬氨酸转氨甲酰酶 （英语：Aspartate carbamoyltransferase， EC 2.1.3.2，简称为 ATCase）是 嘧啶核苷酸 从头合成 中很重要的一个酶，它 催化 的是 天冬氨酸 与 氨甲酰磷酸 生成 氨甲酰天冬氨酸 和 磷酸 的反应。

2022年11月3日 · Carbamoyltransferases (CTases) catalyze carbamoylation, an important modification for the bioactivity of many secondary metabolites with pharmaceutical applications. To generate an iterative CTase, we determine the X-ray structure of GdmN, a one-step CTase involved in ansamycin biosynthesis.