2024年6月6日 · Alzheimer’s Disease (AD) pathology has been increasingly explored through single-cell and single-nucleus RNA-sequencing (scRNA-seq & snRNA-seq) and spatial transcriptomics (ST). However,...

2021年3月1日 · RNA sequencing (RNA-Seq) was carried out on samples from the PREC and VIC brain regions of five AD cases and five controls matched for age, gender, APOE genotype and RIN and selected from a...

2019年5月1日 · Bulk RNA-seq differential analysis was performed by fitting a linear model using the R package limma 53, accounting for the covariates age, RNA integrity number, post-mortem interval, and plate...

We obtained clinical and transcriptomic signatures from the MSBB-AD study of 364 human brains, including whole-transcriptome RNA-seq data from four brain regions (FP, STG, PHG, and IFG) from participants with AD that showed neurocognitive decline as measured by clinical dementia rating (CDR) score > 1 and nondemented controls (CDR = 0).

We present a consensus atlas of the human brain transcriptome in Alzheimer’s disease (AD), based on meta-analysis of differential gene expression in 2,114 postmortem samples. We discover 30 brain coexpression modules from seven regions as the major source of AD transcriptional perturbations.

2020年11月5日 · Alzheimer's disease (AD) is a progressive neurodegenerative disorder of the brain and the most common form of dementia among the elderly. The single-cell RNA-sequencing (scRNA-Seq) and single-nucleus RNA-sequencing (snRNA-Seq) techniques are extremely useful for dissecting the function/dysfunction o …

2023年2月24日 · Our bioinformatic analysis identified discrete glial, immune, neuronal and vascular cell populations spanning Alzheimer’s disease and controls. Astrocytes and microglia displayed the greatest transcriptomic impacts, with the induction of …

2021年1月6日 · We identify three major molecular subtypes of AD corresponding to different combinations of multiple dysregulated pathways, such as susceptibility to tau-mediated neurodegeneration, amyloid-β neuroinflammation, synaptic signaling, immune activity, mitochondria organization, and myelination.

2019年8月9日 · We generated unsorted single-nuclei RNA sequencing data from brain tissue. We leveraged the tissue donated from a carrier of a Mendelian genetic mutation, PSEN1 p.A79V, and two family members who suffer from sporadic AD, but do not carry any autosomal mutations.

2021年5月3日 · Single-cell RNA-sequencing (scRNA-seq) and single-nucleus RNA-sequencing (snRNA-seq) studies have provided remarkable insights into understanding the molecular pathogenesis of Alzheimer's disease. We recently developed scREAD, a database to provide comprehensive analyses of all the existing AD scRNA …