制备人脐静脉内皮细胞和人肺腺癌细胞融合细胞的新方法
VE-cadherin and platelet endothelial cell adhesion molecule-1 (CD31) were highly expressed in HUVECs and HUVEC-A549 cells, but not in A549 cells. Conclusions: These results indicate that HUVEC-A549 cells retain the biological characteristics of human …
Targeting c-Jun in A549 Cancer Cells Exhibits Antiangiogenic Activity
2021年4月9日 · In the present study, we investigated the detailed mechanism of c-Jun in cancer cells to regulate angiogenesis through mediating exosome/miRNA/tensin homolog deleted on chromosome ten (PTEN) signal pathway. A549 cells were obtained from the Cell Resource Center, Peking Union Medical College (Beijing, China).
Interleukin-6 blockade attenuates lung cancer tissue ... - Nature
2017年9月26日 · In this study, we established technologies to generate lung CSC-like cells from human lung cancer cell line A549 by introducing OCT3/4, SOX2 and KLF4, and to construct “lung cancer organoids” in...
制备人脐静脉内皮细胞和人肺腺癌细胞融合细胞的新方法 - PMC
2021年7月28日 · VE-cadherin and platelet endothelial cell adhesion molecule-1 (CD31) were highly expressed in HUVECs and HUVEC-A549 cells, but not in A549 cells. Conclusions: These results indicate that HUVEC-A549 cells retain the biological characteristics of human umbilical vein endothelial cells and A549 cells.
CJ016对人肺癌裸鼠移植瘤的抑制活性研究 - 仁和软件
方法 建立人肺腺癌细胞a549裸鼠移植瘤模型，考察cj016体内抑制肿瘤生长效果；同时将肿瘤进行免疫组化检测，考察其对肿瘤内cd31和细胞凋亡的影响。
IL-17 Promotes Angiogenic Factors IL-6, IL-8, and Vegf ... - Nature
2016年11月7日 · CD31 expression was increased in A549-IL-17 cell-bearing nude mouse tissues. (A) The time course of in vivo growth of A549-IL-17 cells vs. A549-Neo cells in nude mice (n = 5).
在非小细胞肺癌中，肿瘤表达的 B7-H3 促进血管生成模拟物的形成 …
对于体内分析，使用 b7-h3 ko 和模拟编辑的 a549 细胞建立异种移植小鼠模型，并进行免疫组织化学 （cd31） 和组织化学 （过碘酸-希夫，pas） 双重染色以鉴定肿瘤组织中的 vm 和内皮血管
Cyclin-dependent kinase 5 controls vasculogenic mimicry formation in ...
2017年10月21日 · Vasculogenic mimicry (VM) network is present in the lung cancer cell line A549. CDK5 upregulates VM formation of A549 cells both in vitro and in vivo. CDK5 kinase-induced VM in lung cancer cells is accomplished by the …
Epigallocatechin-3-gallate-induced vascular normalization in A549 …
2019年3月27日 · We treated A549 cell (human lung adenocarcinoma cell line) xenograft-bearing nude mice with EGCG in vivo. CD31, αSMA, and collagen IV were labeled and detected using quantum-dot double-labeled immunofluorescence to measure microvessel density, microvessel pericyte-coverage index, and collagen IV expression.
Development of a 3D angiogenesis model to study tumour - Nature
2017年6月7日 · In this study, we describe the development of a novel tri-culture model, using non-small cell lung cancer (NSCLC) cell lines (A549 and Colo699) in combination with a fibroblast cell line (SV 80)...