2024年6月13日 · Scientists have developed α-synuclein PET tracers, but they have limitations (Aug 2023 conference news). One, called F0502B, poorly slipped into the brains of rhesus macaques, while Merck's MK-7337 had off-target binding in monkey cerebella. AC Immune’s ACI-12589 detects α-synuclein aggregates in MSA, but not those in PD, which are much ...

2022年6月12日 · How α-synuclein affected these processes was unknown. To follow up, first author Erinc Hallacli expressed α-synuclein in a yeast model system containing tagged RNA-binding proteins that acted as reporters. α-Synuclein affected the function of several RBPs, particularly those that regulated mRNA degradation.

2024年9月20日 · No inclusions formed when neurons did not express α-synuclein (middle). [Courtesy of Lam et al., Neuron, 2024.] In neurons expressing a piggyBac A53T-α-synuclein transgene the scientists found two different types of aggregate (image below). Type I inclusions contained a lipid-rich mix of vesicular structures and mitochondrial membranes, but ...

2024年2月9日 · For now, this means α-synuclein seeds that can be amplified from the CSF, but other assays might be added as they become validated. Carriers of fully penetrant SNCA mutations are the lone exception to the requirement for a positive α-synuclein seeding test. This is because these rare mutations invariably spark neuronal α-synuclein aggregation.

2017年3月22日 · Behaviorally, α-synuclein KO mice appear largely normal. They display normal reflexes and sensory abilities. They performed similarly to controls in most assays of motor function (e.g., Rotarod, distance travelled in open-field test); however, they reared on hind legs less frequently than wild-type controls.

2002年7月3日 · The transgene consists of the human α-synuclein sequence with the A53T mutation driven by the mouse prion protein (PrP) promoter. The transgene inserted at Chr10:95350683-95399000 (Build GRCm38/mm10), causing a 249 Kb deletion that disrupted the 2310039L15Rik gene, which codes for a lincRNA (Goodwin et al., 2017). Phenotype …

2017年3月22日 · Neurons in the enteric nervous system of the gut develop α-synuclein pathology, but neurons in the brain are spared and there is no evidence of dopaminergic neuron loss (Kuo et al., 2010). Homozygous mice have four copies of the human transgene (two on chromosome 3 and two on chromosome 14) and robust expression in the brain and colon.

2023年1月27日 · The novel findings presented in the two papers, which suggest that normal α-synuclein has functions under physiological conditions, are quite interesting and unexpected. We had reported in 2002 that aggregated α-synuclein, but not the normal soluble species, is exclusively and highly phosphorylated at Ser129 (Fujiwara et al., 2002). Our ...

2022年3月27日 · Mark Cookson National Institute on Aging; Posted: 29 Mar 2022 Finding ways to image α-synuclein pathology in the human brain is a critical need in the neurodegenerative field, as this could allow for both evaluation of disease progression and, potentially, for differential diagnosis between diseases.

2024年11月8日 · Led by Ursula Jakob and Bikash Sahoo, University of Michigan, Ann Arbor, the authors based their conclusions on computer modeling of how polyphosphate binds to α-synuclein, as well as on experiments with recombinant α-synuclein. Forming chains of up to 1,000 molecules long, polyphosphate is found in the brain both inside and outside of neurons.