Plasmid: You have prepared the plasmid pET15b from bacteria in the lab. The plasmid map for pET15b is attached to this problem set. Comment if pET15b plasmid will yield a high or low copy number of plasmids after transformation to bacterial cells. Find a plasmid that can be co-transformed with pET15b without compromising their copy number.

Question: PET15b/KGA-7.3 kb 6xHis Ndel IKGA (1.6 kb) Start LDHUR Bamia LOHRE BamHI PET15b (5.7 kb) LDH-A- 1 kB AmpR Determine the volume of vector and insert to add to a ligation reaction. The concentration of PET15b vector is 25.5 ng/uL and the concentration of LDH insert is 8.3 ng/uL Do the calculation here: a. 5 fmol PET15B vector/25 fmol ...

El plásmido pET15b contiene el promotor T7/lacUV5. Explique cómo se podría inducir la expresión de la proteína beta actina clonada mediante la adición de IPTG a las células que contienen el plásmido pET15b_beta actina.

Answer to Solved The pET15b plasmid contains the T7/lacUV5 promotor. | Chegg.com

E CS Whe he Duo (4643-5500 10 pET-15b A Use the gel and plasmid map to answer this and the next question. Which band corresponds most closely to what you would expect to see when cleaving a PET15b plasmid containing a 900 base pair insert placed between restriction sites Xhol and BamHI, with those respective restrictions en

Question: This is the partial sequence of pET15b plasmid for the expression of a lectin named Calsepa in E. coli. The DNA sequence of the sense (upper) and antisense (lower) are shown with restriction enzyme sites annotated. Please answer the following questions.

Question: 8. (3 pts.) You incubate pET15b plasmid with BamHI and Ndel. Draw what will happen if the BamHI and Ndel are both fully active and their reactions go to completion.

Question: - Commonly used expression plasmid pET15b has the sequence called Amp'. This sequence is necessary for: A) transport of the plasmid into bacterial cell B) stability of the plasmid to bacterial proteases C) ribosome binding to increase protein expression Daffinity purification of the expressed protein E) conferring antibiotic resistance to the transformed

10. If we had cloned OmpR into the pET15b plasmid that contains a T7 promoter with a Lac operator sequence: a. What gene(s) or mutation(s) in Lemo21 (DE3) would be essential for expression of OmpR? (1 marks) b. In order to induce expression of …

Question: You are cloning a gene into the plasmid pet15b (Figure 3A: plasmid map with restriction sites numbered from an arbitrary origin at 0; plasmid is -5.7 kb). PETS 050 Figure 3A Figure 3B A) Design a pair of PCR primers (8 bp of complimentary sequence each, although real ones would be 15-20 bp) to amplify your entire 500 bp gene (partial ...