2023年3月31日 · If the bacteria are Gram-positive cocci in pair, catalase-negative, and α-hemolytic and optochin intermediate, perform the spot bile solubility test. If the bacteria is bile soluble, report it as Streptococcus pneumoniae.

2022年8月10日 · This test determines whether the bacterium is either sensitive (susceptible) to optochin or resistant to the chemical. The optochin test is widely used in the form of filter paper discs, impregnated with ethylhydrocupreine hydrochloride, which are applied directly to inoculated plates before incubation.

2013年5月17日 · Optochin sensitivity allows for the presumptive identification of alpha-hemolytic streptococci as S. pneumoniae, although some pneumococcal strains are optochin-resistant.

2022年8月10日 · Optochin sensitivity test is an important parameter used to identify Streptococcus pneumoniae from other α-hemolytic streptococci species in a laboratory setting. The main purpose of this test is to determine the effect of optochin on microorganisms.

2022年6月12日 · This test determines whether the bacterium is either sensitive (susceptible) to optochin or resistant to the chemical. How is optochin susceptibility determined? Susceptibility to optochin is determined by placing an optochin-impregnated disk on a nutrient agar plate seeded with the microbe under investigation.

A positive result is reported if a zone radius (on a 6 mm disc) is >8 mm. Optochin sensitivity can be used in primary plates from specimens (e.g. sputum) likely to be contaminated by oral flora to differentiate suspected pneumococci from other alpha-haemolytic streptococci.

Optochin (Opt) susceptibility is used largely for the identification of Streptococcus pneumoniae in diagnostic laboratories. Opt-resistant (Opt r) S. pneumoniae isolates have been reported, however, indicating the potential for misidentification of this important pathogen.

Optochin susceptibility test is a key diagnostic tool used to differentiate Streptococcus pneumoniae from other alpha-hemolytic streptococci, particularly Streptococcus viridans group. The test is based on the sensitivity of S. pneumoniae to the …

2021年6月1日 · Optochin is a chemical used as an antibiotic that interferes with the activity of enzyme ATPase and production of ATP in microorganisms. It helps in the presumptive identification of alpha-hemolytic Streptococcus pneumoniae (sensitive to Optochin) from other alpha-hemolytic streptococcal species (resistant to Optochin), although some ...

2024年6月9日 · In 1955, Bowen and Jeff proposed a method to differentiate S. pneumoniae from other viridans Streptococci by impregnating a disk with optochin. This method is known as the optochin susceptibility test and is used to identify pneumococcus bacteria. To distinguish S. pneumoniae from other α-hemolytic (viridans) Streptococci.