Bacterial vector for expression of N-terminally 6xHis-tagged proteins with a thrombin site. For other reading frames, use pET-28b(+) or pET-28c(+).

pET-28a-c(+)载体带有一个N端的His/Thrombin/T7蛋白标签，同时含有一个可以选择的C端His标签。 pET28a载体的单一的多克隆位点见上面的环状质粒图谱。 注意：载体序列是以pBR322质粒的编码规矩进行编码的，所以T7蛋白表达区在质粒图谱上面是反向的。

pET-28a(+) is a bacterial expression vector designed for the production of N-terminally 6xHis-tagged proteins with a thrombin cleavage site. This vector is particularly suitable for proteins that require post-translational processing to remove the His-tag.

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pET-28a(+) is a bacterial expression vector designed for the production of N-terminally 6xHis-tagged proteins with a thrombin cleavage site. This vector is particularly suitable for proteins that require post-translational processing to remove the His-tag.

pET系列载体是目前应用最广泛的重组蛋白表达载体，载体构建时目的片段被克隆入载体噬菌体T7转录翻译系统下，经转化在宿主T7RNA聚合酶的诱导下进行表达。 T7RNA聚合酶具有超强并特异的启动T7启动子基因表达功能，当其被完全诱导时，可使宿主本身的表达几乎全部转化为目的基因的表达，诱导几个小时后即可使得最终目的基因表达产物超过细胞总蛋白的50%。 pET-28a载体带有一个N端的His/Thrombin/T7蛋白标签，同时含有一个可以选择的C端His标签。 pET28a载 …

pET-28a(+)是一种常用的融合蛋白类型原核高效表达载体,含有抗卡那霉素基因。 表达由宿主细胞提供的T7 RNA聚合酶诱导。 宿主菌:建议克隆用E.coli DH5α 或TOP10 做受体菌;表达用E.coli BL21(DE3)或BL21(DE3)pLysS做受体菌。

The pET System is the most powerful system yet developed for the cloning and expression of recombinant proteins in E. coli. Target genes are cloned in pET plasmids under control of strong bacteriophage T7 transcription and (optionally) translation signals; expression is induced by providing a source of T7 RNA polymerase in the host cell.

The pET-28a-c(+) vectors carry an N-terminal His•Tag®/ thrombin/ T7•Tag® configuration plus an optional C-terminal His•Tag sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so …

Plasmid pET-28a(+)::NL from Dr. Andrew Millar's lab contains the insert NLUC and is published in Plant Methods. 2019 Jul 8;15:68. doi: 10.1186/s13007-019-0454-4. eCollection 2019. This plasmid is available through Addgene.