Bacterial vector that encodes a signal sequence for inducible expression of proteins in the periplasm. Sequence Author : MilliporeSigma (Novagen) Open in SnapGene

The pET-22b(+) vector (Cat. No. 69744-3) carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal His•Tag® sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map.

Provide pET-22b(+) vector/plasmid map, full length sequence, antibiotic resistance, size and other information

The pET-22b(+) vector carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal His•Tag® sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB038). Source: Sigma-Aldrich .

(5410) BlpI (5332) BmeT110I (5331) AvaI - BsoBI - PaeR7I - PspXI - XhoI (5323) EagI - NotI (5316) HindIII (5310) SalI (5307) SacI (5305) Eco53kI (5297) EcoRI (5291) BamHI (5269) NcoI (5268) MscI (5235) BseRI (5221) BfuAI - BspMI (5203) NdeI (5163) XbaI lac repressor encoded by lacI binding site

Blp l Bgl ll pET-22b(+) 5.5kb EcoR l A m p R RBS Xba l Xho l Not l Hind lll Sal l Sac l BamH l Nco l Nde l T7 terminator pelB leader His tag T7 promoter lac O M C S L a c l

Analyze a DNA sequence to see restriction sites and map. Vector Database. Browse a digital-only collection of vector backbone information

Provide pET22b-EGFP vector/plasmid map, full length sequence, antibiotic resistance, size and other information

Provide pET22b-mCherry vector/plasmid map, full length sequence, antibiotic resistance, size and other information

Analyze a DNA sequence to see restriction sites and map. Vector Database. Browse a digital-only collection of vector backbone information