You have cloned a 1 kb gene into the pET17b vector (see included map on the next page) using an HindIII site before the gene and an EcoRV site after the gene. You have confirmed that the 1 kb gene you cloned into the pET17b vector does NOT contain any sites for the restriction enzyme SpeI. a. Based on the provided map, if you digest the resulting

You have cloned a 1 kb gene into the pET17b vector (see included map on the next page) using an HindIII site before the gene and an EcoRV site after the gene. You have confirmed that the 1 kb gene you cloned into the pET17b vector does NOT contain any sites for the restriction enzyme SpeI. a. (1 point) Based on the provided map, if you digest the

You add 15 ng of template DNA (the pET17b plasmid containing the gene is 5904 bp) to the PCR reaction. After 33 cycles of PCR, what is the theoretical yield in number of DNA fragments?(6.02*1023 bp/molbp, 660 g/molbp) Round your answer to …

Question: You are designing PCR primers to amplify a gene using that gene cloned into the pET17b plasmid as a template. The coding strand of DNA seen below represents the gene (bold) in the vector you are amplifying it out of.

Design suitable primers for the PCR: a) to clone the sequence coding for the native form of the protein between Ndel and Xhol; b) to fuse the protein to the His tag contained in PET17b, by cloning the sequence coding for it between HindIII and Xhol. Write both primers in the 5'-3' direction. Detail your answer.

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2017年10月26日 · Biology archive containing a full list of biology questions and answers from October 26 2017.