Oligonucleotide synthesis is the chemical synthesis of relatively short fragments of nucleic acids with defined chemical structure (sequence). The technique is extremely useful in current …

These robust reactions allow both chemists and molecular biologists to easily generate specific ribo- and deoxyribo oligonucleotides with a variety of labels, modified linkages, and …

2015年12月18日 · Refined oligonucleotide synthesis method. After a solid support containing the correct initial nucleotide (or modification) has been placed on the synthesis platform, IDT …

Phosphoramidite chemistry, developed in the 1980s and later enhanced with solid-phase supports and automation, is the method of choice for DNA oligonucleotide manufacturing. Opposite of …

Phosphoramidite oligo synthesis proceeds in the 3'- to 5'-direction (opposite to the 5'- to 3'-direction of DNA biosynthesis in DNA replication). One nucleotide is added per synthesis …

2024年6月18日 · Controlled enzymatic synthesis is a method for the de novo production of oligonucleotides that combines elements of solid-phase synthesis and of enzymatic …

DNA oligonucleotides are synthesized in a 3′–5′ manner by coupling acid-activated deoxynucleoside phosphoramidites to an initial deoxynucleoside attached to a solid support …

In this perspective, we briefly review the techniques and technologies that enable the synthesis of DNA oligonucleotides and their assembly into larger DNA constructs with a focus on recent …

Oligonucleotide therapeutics are emerging as a powerful platform for modulating gene expression across a range of diseases and applications. However, their complex chemical synthesis, …

De novo oligonucleotide synthesis (oligo synthesis) is the synthesis of defined sequences of single-strand nucleic acids. Its early efforts could be found out in the 1950s, followed by large …