Mycoplasmas are frequent contaminants of cell cultures and bioprocessing fluids. It is well established that mycoplasma contamination of continuous cell cultures ranges from 15-35%, with primary cell cultures exhibiting a minimum 1% contamination rate.

2020年10月8日 · Mycoplasma contamination? Find out where it comes from, how to detect it, prevent it, and remove it from your tissue culture experiments.

In many cases there are no signs of mycoplasma contamination, however, mycoplasma can cause changes in cell growth characteristics, inhibition of cell metabolism, disruption of nucleic acid synthesis, chromosomal abberations, changes in cell membrane antigenicity, and can alter transfection rates and virus susceptability.

Learn what causes mycoplasma contamination in cell cultures and how to detect, remove, and prevent future contamination of these detrimental agents.

2017年3月1日 · It is there, but doesn’t present the usual signs of contamination. The mycoplasma doesn’t overtake the cells or kill them, it lives in the culture and many times goes completely undetected. Mycoplasma was first detected in cell culture in 1956, so why is it still such an issue?

2020年12月14日 · What are mycoplasma contamination signs in my culture? The nasty thing about mycoplasma contamination is, without specific testing, it will stay unnoticed. Mycoplasma contaminations are not visible to the naked eye.

Read on for tips about what to do to prevent mycoplasma contamination, how to test for it, and what to do in the unfortunate event that your cells become contaminated. Preventing Mycoplasma Contamination. The best possible way to deal with mycoplasma …

There are several different techniques to identify if your cell cultures are contaminated with mycoplasma. These include histochemical staining, ELISA, DNA fluorochrome staining, microbiological culture, biochemical / bioluminescence methods, and PCR.

Check for Mycoplasma contamination (Staining method, cultivation or ELISA). If mycoplasma positive, check methods (positive and negative controls and evaluate). If required, repeat treatment with increased MycoXpert concentrations (Dilution 2, Dilution 3 or Dilution 4).

Signs of Mycoplasma Contamination in Cell Culture. The following symptoms may indicate mycoplasma contamination: 1. A once vigorous cell line grows poorly. 2. An adherent cell line is less adherent. 3. Cell line cannot be grown to high density (>1 × 106 cells/ml). 4. B or T hybridomas fail to be generated from cell fusion techniques. 5.