Crosslinking reagents (or crosslinkers) are molecules that contain two or more reactive ends capable of chemically attaching to specific functional groups (primary amines, sulfhydryls, etc.) …

In chemistry and biology, a cross-link is a bond or a short sequence of bonds that links one polymer chain to another. These links may take the form of covalent bonds or ionic bonds and …

In recent times, chemical crosslinking has emerged as an efficient tool for the study of biomolecules like proteins. It finds its application in various studies including the attachment of …

2017年12月21日 · Harnessing proximity-enabled reactivity of an unnatural amino acid incorporated in the bait toward a target residue of unknown proteins, here we genetically …

Chemical cross-linking and mass spectrometry (CXMS) analysis has recently become a powerful method in protein structural elucidation, due to its high sensitivity and throughput, its ability to …

Chemical crosslinking is an approach that connects polymer backbones through chemical bonds [56,119]. It is possible to significantly improve the mechanical strength and chemical stability of …

Chemical cross-linking is a powerful biochemical technique used to investigate protein-protein interactions, study protein structures, and gain insights into the functional aspects of proteins. …

2018年10月29日 · Over the past decade, cross-linking mass spectrometry (CLMS) has developed into a robust and flexible tool that provides medium-resolution structural information. CLMS …

Chemical cross-linking coupled with mass spectrometry is emerging as a complementary technique to traditional structural biology methods and can provide low-resolution structural …

In this article, we discuss the critical steps of chemical cross-linking and its implications for (structural) biology: reagent design and cross-linking protocols, separation and mass …