2024年4月12日 · Phenotypic methods recommended for the detection of MRSA include: Cefoxitin broth microdilution. Oxacillin broth microdilution. Cefoxitin disk diffusion testing. A screening agar containing 6 μg/ml of oxacillin in Mueller-Hinton agar supplemented with NaCl (4% w/v; 0.68 mol/L) is an alternative method of testing for MRSA.

It is being recommended for detection of methicillin resistance in Staphylococcus aureus (MRSA) when using disk diffusion testing. The aim of our study was to evaluate the efficacy of cefoxitin disc diffusion test to characterize MRSA and compare it with oxacillin agar screening and detection of mecA gene by PCR.

Recent studies have indicated that disc diffusion testing by using the cefoxitin disc is far superior to most of the currently recommended phenotypic methods like oxacillin disc diffusion and oxacillin screen agar testing and that it is now an accepted method for the detection of MRSA by many reference groups including CLSI (18).

2023年6月14日 · On the other hand, the use of cefoxitin is very sensitive and specific as mec-A mediated marker to recognize methicillin resistance. 15 Detection of antimicrobial resistance can also be done by using chromogenic agar media.

Routine oxacillin disk diffusion tests often fail to detect heterogeneous MRSA populations. In the present study, a recently proposed disk diffusion method that employs a cephamycin antibiotic (cefoxitin 30 microg; BD Sensi-disc, Becton Dickinson, Germany) was evaluated using 155 clinical isolates of S. aureus (73 mecA positive and 82 mecA ...

2003年9月9日 · Evaluation of three techniques for detection of low level methicillin resistant Staphylococcus aureus (MRSA): a disk diffusion method with cefoxitin and moxalactam, the Vitek 2 system, and the MRSA‐screen latex agglutination test

2009年1月1日 · The recommended breakpoints for the cefoxitin disk diffusion test for Staphylococcus aureus were recently modified. In this large-sample study, cefoxitin sensitivity and specificity compared to those of oxacillin were 97.3% and 100%, respectively.

2004年4月27日 · The cefoxitin disk diffusion test was found to be 100% sensitive and specific for MRSA detection at a 30°C incubation temperature for 24 h. This implies that the cephamycin disk test is a preferable alternative to the oxacillin disk diffusion method for routine MRSA screening.

2023年6月30日 · This study evaluates the effectiveness of cefoxitin disk diffusion as a method to detect methicillin-resistant Staphylococcus aureus (MRSA), comparing with HiCrome MRSA screening agar and PCR-based mecA gene detection.

2019年7月1日 · The Yellowish colony confirmed with gram staining and tested using Staphytect plus Test DR 850 M. Identified of MRSA using cefoxitin disc diffusion test and dupleks PCR. This research reported cefoxitin-RSA was detected in 41,67% of …