2016年5月1日 · Use of modernistic approach such as membrane filtration, reverse micellar systems, aqueous two phase extraction and chromatographic techniques have shown promise in this regard. Besides its...

2013年6月25日 · The present work mainly focuses on optimization of conditions for extraction of bromelain from pineapple (Ananas comosus L.) core and purification of bromelain by reverse micellar extraction (RME) and precipitation techniques. Extraction conditions like type of buffer, pH, temperature and strength of buffer on bromelain activity and protein ...

2023年7月11日 · Bromelain is a mixture of cysteine endopeptidase usually extracted from pineapple juice and is used for the treatment of various human diseases and in industrial applications. Bromelain demand is quickly increasing, and its recovery from pineapple residues appears to be a sustainable waste management strategy.

Bromelain is an effective chemoresponsive proteolytic enzyme derived from pineapple stems. It contains several thiol endopeptidases and is extracted and purified via several methods. It is most commonly used as an anti-inflammatory agent, though scientists have also discovered its potential as an anticancer and antimicrobial agent.

Bromelain can be purified from crude pineapple extract using membrane processes. In fact, membrane processes are considered the most attractive purification technology since they offers major advantages such as high throughput of product, scale up facilities due to the modular character, environmentally friendly, cost effective ( Nor et al ...

Use of modernistic approach such as membrane filtration, reverse micellar systems, aqueous two phase extraction and chromatographic techniques have shown promise in this regard. Besides its industrial applications, bromelain has been widely utilized as a potential phytomedical compound.

This study examines the extraction of bromelain from pineapple peels by using an aqueous two-phase system (ATPS). Bromelain from the crude extract predominantly partitioned to the polyethylene glycol …

2022年11月14日 · Thus, in this study, bromelain is extracted from the flesh, crown, stem, core and peel of pineapples using simple one stage hollow fiber nanofiltration membrane process. The different parts of the pineapple were crushed to extract the juice. The juice was then centrifuged and the supernatant was then passed through a nanofiltration membrane.

2023年1月4日 · The review focuses on homogenous and heterogenous techniques used for the estimation of Bromelain in the crude extract. This review also emphasizes on separation novel techniques, such as ion-exchange chromatography for isolation and purification of Bromelain supported by Gel Chromatography.

Bromelain is a complex combination of multiple endopeptidases of thiol and other compounds derived from the pineapple fruit, stem and/or root. Fruit bromelain and stem bromelain are produced completely distinctly and comprise unique compounds of enzymes, and the descriptor “Bromelain” originally referred in actuality to stem bromelain.