If the ratio is <2, HER2 gene amplifi cation was not observed. The result is negative. If the ratio is ≥2, HER2 gene amplifi cation was observed. The result is positive. A ratio at or near the cut-off (1.80 - 2.20) should be interpreted with caution. Leica HER2 FISH - Interpretation of Breast Specimens Cell Line Leica BondTM Oracle

2024年3月28日 · A HER2 FISH (Fluorescence In Situ Hybridization) test is done on breast cancer tissue removed during biopsy to see whether the cells have extra copies of the HER2 gene.

2024年6月27日 · FISH testing can tell your doctor whether you have this type of cancer, called HER2 -positive breast cancer, or a HER2-negative cancer with normal levels of the protein. At the time of a...

2024年6月7日 · In situ hybridization (ISH) assay is used to detect HER2 gene amplification, generally using a dual probe fluorescent ISH assay (D-FISH) using fluorochrome labeled probes for (a) the HER2 locus on the long arm of chromosome 17 and (b) a site near the centromere of chromosome 17 (CEN17 or CEP17)

HER2 amplification is found in about 15 – 25% of breast cancers. HER2 amplification is present in about 7 – 34% of gastric cancers. Genetics Amplification of HER2 is generally a new mutation. Indications for Ordering Detect amplification of the HER2 gene associated with breast and gastric cancer prognosis and treatment decisions.

Breast tumors with an equivocal HER2 IHC result need to undergo reflex testing by FISH to resolve the HER2 status, allowing clinical decisions to be made about adjuvant treatment. Breast cancers with diffuse intense circumferential membrane staining (so called “chicken-wire” pattern) in >10% of invasive tumor cells.

This test uses fluorescence in situ hybridization (FISH) to detect HER2 (also known as ERBB2 or HER-2/neu) oncogene amplification in the invasive component of a breast cancer specimen. This test may be used in patients with diagnosed breast …

2019年11月3日 · Although HER2 status can be directly tested by in situ hybridization (ISH), many laboratories have adopted IHC as a screening test, and FISH as a confirmation test for HER2 IHC equivocal cases, considering higher failure rate, longer procedure time and higher reagent cost of FISH, compared to that of IHC.

2024年4月2日 · Most people with breast cancer will undergo tests to determine whether their cancer is HER2-positive or HER2-negative. It’s important to find out this information as early as possible because your HER2 status tells you and your doctor which treatments are …

HercepTestTM is a semi-quantitative immunohistochemical assay to determine HER2 protein overexpression in breast cancer tissues routinely processed for histological evaluation and formalin-fixed, paraffin-embedded cancer tissue from patients with adenocarcinoma of the stomach, including the gastroesophageal junction*.