For quantitative rt-PCR, 10 ng cDNA was used. Forward and reverse primers for all CYPs tested, and endogenous references, Hypoxanthine phosphoribosyltransferase 1 (HPRT1) and GAPDH, were purchased ...

The study presents compelling evidence that the melanocortin system originating in the arcuate nucleus of the hypothalamus plays a crucial role in puberty onset, representing a significant advance in ...

The loss of one hypoxanthine molecule leads to the loss of one IMP molecule. Consequently, if the PPP is utilized to synthesize IMP, which requires up to seven ATP molecules, the overall ATP balance ...

All experimental procedures were performed by following the rules of the Brazilian Guidelines for Animal Experimentation of the National Council for the Control of Animal Experimentation (CONCEA) and ...