A restriction map of the 5.3 kb insert is prepared. The recombinant plasmid is digested with the enzymes listed above in single digests, and then several combinations of enzymes are tested in double ...

Figure 3: 1% agarose gel tracing the large-scale purification of pBGS19Luxwt plasmid DNA. Anion-exchange chromatography is commonly used for final purification of plasmid DNA 13. It was found that ...