Ago proteins constitute a key component of the RNA-induced silencing complex (RISC), contributing to both the architectural and catalytic functionalities associated with siRNA guide strand selection ...

The siRNA associates with cellular proteins to form the RNA-induced silencing complex (RISC). The siRNA antisense strand guides RISC to the target mRNA, resulting in endonucleolytic cleavage.

Gene silencing techniques Description of mechanism Ref. siRNA The siRNA associates with RISC leading to unwinding of the dsRNA, sense strand of which is cleaved by Argonaut2 protein. The antisense ...

Viroid replication generates dsRNA intermediates, which are processed by Dicer into 21- to 25-nucleotide siRNAs. These siRNAs are then incorporated into siRNA – ribonuclease complexes (RISC). If the ...

Evidence has also shown that siRNA is involved in antiviral defense ... of the target mRNA and interaction between the siRNA and RISC. Information provided by studies such as these may lead ...