PCR involves the enzymatic amplification of nucleic acid templates, and can be divided into four major steps, listed below. 1 Denaturation: Double-stranded DNA separates into single strands. Annealing ...

Each cycle of PCR has three main steps, as described in the following sections. The number of new copies of the DNA sequence of interest doubles with each three-step cycle. Thus, if the PCR ...

The PCR machine increases and decreases the temperature of the sample in automatic, programmed steps. Initially, the mixture is heated to denature, or separate, the double-stranded DNA template ...

PCR amplifies DNA in a three-step process: denaturation, which melts double-stranded DNA into single strands; annealing, which lets small pieces of primer DNA bind to either side of the region of ...

Coamplification at lower denaturation temperature (COLD)-PCR is a PCR-based application that preferentially enriches the amplification of mutants and variants throughout the course of ...