Prepare a polyacrylamide minigel of the desired concentration containing 8 M urea (for example, Bio-Rad Mini ... Rapidly add 300 μl of ice-cold nuclease S1 digestion buffer and immediately ...

For example, the commonly used Cas9 is about 1,400 amino acid residues long ... The team observed a 4.4-fold increase in the editing efficiency of this modified nuclease, surpassing most of the ...