In vivo perturbations and single-cell RNA-seq reveal cell-type-specific STAT1-IFNg signaling in regulation of trained immunity in tissue-resident immune cells.

Handle tissues gently. Grasp and cut samples at the edges, so the areas of interest aren't damaged. At right is a section of mouse spleen damaged by rough handling. Many of the cells are distorted ...

If you genetically alter mice to have an absence of the thyroid hormone T4, their spleen size is drastically reduced, but this effect is actually reversible with an injection of T4." ...