Without the second gene copy to worry about, we can generate these knockout cell lines in a fairly streamlined and convenient fashion — and we can hit pretty much any gene in the genome.

The company uses knock-out cell lines to eliminate doubts about cross reactivity, and has recently introduced biophysical quality-control methods, such as mass spectroscopy, high-performance ...

The Transgenic and Knockout Core can develop mice with targeted mutations in the entire mouse or in specific tissues or cell types. It normally takes about a year to generate a gene-targeted mouse.

Many methods have been suggested for trajectory inference. However, in this setting, it is often unclear how one should handle multiple biological groups or conditions, e.g. constructing and comparing ...

In the Transgenic and Knockout Core, transgenic mice are generated by pronuclear microinjection of foreign DNA fragments into one-cell-stage mouse embryos. On average, it takes about three to four ...

For B cell activation experiments, mice were immunized with DNP-Ficoll and sacrificed 7 days later. Results IL27R knockout mice showed a decreased CIA arthritis score compared to wild type controls.

Objectives: Develop a new drug that inhibits both pDC and B-cell functions for the treatment of autoimmune diseases. Methods: An anti-BDCA2 antibody was generated from mouse hybridoma and humanized by ...