RNA cleavage can occur either: (i) by a transesterification mechanism that yields 2’,3’-cyclic-PO 4 and 5’-OH ends ... The Aspergillus, Coccidioides, and Candida Trl1 enzymes all strongly prefer GTP ...

The assay is run in two main steps, the kinase reaction step followed by the detection step with HTRF reagents (Fig. 1). The phosphorylation of the biotin-tagged generic peptide is initiated by ...