Using three different mutagenesis strategies coupled with a cell-surface binding assay, the authors identified a number of residues in the Frizzled 8 CRD that, when mutated, disrupt Wnt binding ...

Frizzled (FZD) proteins are the principal receptors of the Wnt signaling pathway. However, whether Wnt ligands induce FZD endocytosis and degradation remains elusive. The transmembrane E3 ubiquitin ...