There are four different categories of restriction enzymes. Type I restriction enzymes cut DNA at random locations far from their recognition sequence, type II cut within or close to their ...

Most restriction sites are 4 to 6 bases long, and most are palindromic, meaning that the sequence reads the same forward and backward. HindIII, for example ... enzymes: type I, which recognize ...

“In the early days, you couldn’t do much with DNA because it was a very large molecule,” Roberts recalled. “As soon as the Type II restriction enzymes were discovered—and HindII in particular—it was ...

For this reason, scientists looked for ways of improving the accuracy of restriction enzymes and of modifying them so that they could recognize a sequence that is unique in the genome. Meganucleases ...