Many DNA polymerases also have an additional intrinsic proofreading exonuclease activity. The high accuracy or fidelity of semi-conservative DNA replication is the product of nucleotide template ...

They found that the most common pattern was for the activity to be the same at all three observational points, whether the enzymatic period was during exonuclease repair or DNA extension. “This ...

6 A combination of highly processive thermostable DNA polymerase and a high-fidelity polymerase with 3′-5′ exonuclease activity helps achieve long-range PCR. 7 This allows the amplification of long ...

Briefly, this technique relies on the use of the non-specific endo-exonuclease micrococcal nuclease, an enzyme derived from the bacteria Staphylococcus aureus, to bind and cleave protein-unbound ...