All of the information needed to build and maintain an organism — whether it's a human, a dog, or a bacterial cell — is contained in its DNA. DNA molecules are composed of four nucleotides ...

Polymerase chain reaction ... and end of the DNA target. Then, to perform PCR, the DNA template that contains the target is added to a tube that contains primers, free nucleotides, and an enzyme ...

PCR, or Polymerase Chain Reaction ... single-stranded DNA template. The optimal annealing temperature depends on the primer sequence and length. The temperature is raised to around 72 °C, which is the ...